L-glutamine supplementation reduced morphological damage in the renal glomerulus of rats with Walker-256 tumor.

PURPOSE: To evaluate the effects of the experimental subcutaneous Walker-256 tumor and L-glutamine supplementation, an antioxidant, on the glomerular morphology of rats. METHODS: Twenty Wistar rats were distributed into four groups (n = 5): control (C); control treated with 2% L-glutamine (CG); rats with Walker-256 tumor (WT); and rats with Walker-256 tumor treated with 2% L-glutamine (WTG). Renal histological samples were submitted to periodic acid-Schiff and Masson's Trichrome staining to analyze glomerular density, morphometry of glomerular components and glomerulosclerosis; and to immunohistochemistry for fibroblast growth factor-2 (FGF-2). RESULTS: WT showed 50% reduction in body mass gain and cachexia index > 10%, while WTG demonstrated reduction in cachexia (p < 0.05). WT revealed reduction of glomerular density, increase in the glomerular tuft area, mesangial area, matrix in the glomerular tuft, decrease in the urinary space and synechia, and consequently higher glomerulosclerosis (p < 0.05). L-glutamine supplementation in the WTG improved glomerular density, and reduced glomerular tuft area, urinary space, mesangial area, and glomerulosclerosis compared to WT(p < 0.05). WT showed higher collagen area and FGF-2 expression compared to C (p < 0.05). WTG presented lower collagen fibers and FGF-2 expression compared to WT (p < 0.05). CONCLUSIONS: L-glutamine supplementation reduced cachexia and was beneficial for glomerular morphology of the rats, as well as it reduced kidney damage and improved the remaining glomeruli morphology.

L-glutamine supplementation reduced morphological damage in the renal glomerulus of rats with Walker-256 tumor

ABSTRACT Purpose: To evaluate the effects of the experimental subcutaneous Walker-256 tumor and L-glutamine supplementation, an antioxidant, on the glomerular morphology of rats. Methods: Twenty Wistar rats were distributed into four groups (n = 5): control (C); control treated with 2% L-glutamine (CG); rats with Walker-256 tumor (WT); and rats with Walker-256 tumor treated with 2% L-glutamine (WTG). Renal histological samples were submitted to periodic acid-Schiff and Masson's Trichrome staining to analyze glomerular density, morphometry of glomerular components and glomerulosclerosis; and to immunohistochemistry for fibroblast growth factor-2 (FGF-2). Results: WT showed 50% reduction in body mass gain and cachexia index > 10%, while WTG demonstrated reduction in cachexia (p < 0.05). WT revealed reduction of glomerular density, increase in the glomerular tuft area, mesangial area, matrix in the glomerular tuft, decrease in the urinary space and synechia, and consequently higher glomerulosclerosis (p < 0.05). L-glutamine supplementation in the WTG improved glomerular density, and reduced glomerular tuft area, urinary space, mesangial area, and glomerulosclerosis compared to WT(p < 0.05). WT showed higher collagen area and FGF-2 expression compared to C (p < 0.05). WTG presented lower collagen fibers and FGF-2 expression compared to WT (p < 0.05). Conclusions: L-glutamine supplementation reduced cachexia and was beneficial for glomerular morphology of the rats, as well as it reduced kidney damage and improved the remaining glomeruli morphology.

Combined use of systemic quercetin, glutamine and alpha-tocopherol attenuates myocardial fibrosis in diabetic rats.

This study aimed to analyze the effects of the quercetin (100 mg/kg), 1% glutamine and 1% α-tocopherol antioxidants in the myocardium of rats with streptozotocin-induced diabetes mellitus. Twenty male rats were subdivided into four groups (n = 5): N (normoglycemic); D (diabetic); NT (normoglycemic treated with antioxidants); and DT (diabetic treated with antioxidants) treated for 60 days. Clinical parameters, oxidative stress markers, inflammatory cytokines, myocardial collagen fibers and immunoexpression of superoxide dismutase 1 (SOD-1), glutathione peroxidase-1 (GPx-1), interleukin-1ß (IL-1-ß), transforming growth factor-beta (TGF-ß), and fibroblast growth factor-2 (FGF-2) were evaluated. Results showed reduced body weight, hyperphagia, polydipsia and hyperglycemic state in groups D and DT. The levels of glutathione (GSH) were higher in NT and DT compared to N (p < 0.01) and D (p < 0.001) groups, respectively. Greater GSH levels were found in DT when compared to N animals (p < 0.001). In DT, there was an increase in IL-10 in relation to N, D and NT (p < 0.05), while GPx-1 expression was similar to N and lower compared to D (p < 0.001). TGF-ß expression in DT was greater than N (p < 0.001) group, whereas FGF-2 in DT was higher than in the other groups (p < 0.001). A significant reduction in collagen fibers (type I) was found in DT compared to D (p < 0.05). The associated administration of quercetin, glutamine and α-tocopherol increased the levels of circulating interleukin-10 (IL-10) and GSH, and reduced the number of type I collagen fibers. Combined use of systemic quercetin, glutamine and alpha-tocopherol attenuates myocardial fibrosis in diabetic rats.

Uma análise das teleconsultorias assíncronas em saúde auditiva do Núcleo de Telessaúde do Rio Grande do Norte / An analysis of asynchronous hearing health teleconsulting at the Rio Grande do Norte Telehealth Center

RESUMO Objetivo identificar o perfil das teleconsultorias assíncronas na área da saúde auditiva do Núcleo Técnico Científico de Telessaúde do Rio Grande do Norte (RN). Métodos este estudo é retrospectivo e descritivo. Foram analisados os registros das teleconsultorias de janeiro de 2015 a dezembro de 2019 na plataforma de teleconsultoria do Núcleo de Telessaúde do RN. As teleconsultorias foram filtradas e categorizadas por dois fonoaudiólogos quanto a estado de origem, gênero e profissão do solicitante, tema e objetivo da questão. Resultados entre as teleconsultorias realizadas no período, foram incluídas, neste estudo, as teleconsultorias na área da saúde auditiva. Em ordem decrescente de frequência, os profissionais solicitantes foram: agentes comunitários de saúde, fonoaudiólogos, agentes de combate a endemias, médicos, enfermeiros, agentes de saúde pública, técnicos e auxiliares de enfermagem e outros. Quanto aos objetivos das teleconsultorias, de maior a menor frequência, foram constatadas perguntas sobre condutas, avaliação, tratamento, relações entre fatores, etiologias, prevenção, sintomas, implantação, acesso ao sistema de saúde e outras. Em relação às temáticas, em ordem descendente, foram observadas perguntas sobre hipoacusia, dispositivos auxiliares de audição, zumbido, otite, programa saúde na escola, emissões otoacústicas, otalgia, labirintite e perfuração timpânica. Conclusão as teleconsultorias assíncronas sobre saúde auditiva tiveram maior frequência por solicitantes do gênero feminino, agentes comunitários de saúde e fonoaudiólogos, sobre a temática da hipoacusia e de dispositivos auxiliares de audição, com objetivo de tomada de decisões para condutas e avaliação na área da saúde auditiva.

ABSTRACT Objective To identify the profile of asynchronous hearing health teleconsulting ​​ at the Scientific-Technical Center for Telehealth in Rio Grande do Norte (RN). Methods This study is retrospective and descriptive. Teleconsulting records from January 2015 to December 2019 on the telehealth platform of the Telehealth Center of RN were analyzed. The teleconsultations were filtered and categorized by two speech-language therapists regarding the applicant's state of origin, gender and profession, theme and objective of the question. Results Among the teleconsultations carried out in the period, the ones regarding the hearing health area were included in this study. In decreasing order of frequency, the requesting professionals were: community health agents, speech-language therapists, endemic disease control agents, doctors, nurses, public health agents, nursing technicians and assistants and others. As for the objectives of teleconsulting, from higher to lower frequency, were identified questions about: conduct, evaluation, treatment, relations among factors, etiologies, prevention, symptoms, implementation, access to the health system and others. Concerning the themes, in descending order, were observed questions about hearing loss, hearing aid devices, tinnitus, otitis, school health program, otoacoustic emissions, otalgia, labyrinthitis and tympanic perforation. Conclusion Asynchronous teleconsulting on hearing health was more frequent on female applicants, community health agents and speech-language therapists, concerning the theme of hearing loss and hearing aid devices, to make decisions for conducts and evaluation in the health area hearing.

Reprodutibilidade de testes de resolução temporal em adultos / Reproducibility of temporal resolution tests in adults / Reproducibilidad de pruebas de resolución temporal en adultos

O uso de testes comportamentais é uma prática clínica frequente na audiologia devido à sua grande contribuição ao diagnóstico e aos processos de intervenção fonoaudiológica. Objetivo: Verificar a reprodutibilidade dos protocolos de avaliação da resolução temporal em adultos. Método: Participaram da amostra 34 sujeitos, 22 do sexo feminino e 12 do masculino, com média de idade de 26,21 anos (20 a 52 anos; dp= 8,92) seguindo os critérios: ausência de histórico otológico e/ou audiológico e queixas escolares; normalidade no padrão audiológico e no teste dicótico de dígitos. Foram utilizados na pesquisa os testes Random Gap Detection Test e Gap in Noise, a 50 dB. Ambos foram aplicados em dois momentos, sendo a segunda aplicação com intervalo de uma semana da primeira. O teste Wilcoxon foi utilizado para análise do desempenho da amostra no teste GIN em função da orelha e Teste de Friedman para análise do RGDT em função da frequência testada nos dois momentos. Foi adotado o nível de significância de 5%. O coeficiente de correlação intraclasse foi utilizado na análise da concordância entre as aplicações teste(T1) e reteste (T2) pelo mesmo avaliador (reprodutibilidade). Resultados: Não houve diferença entre as frequências testadas no RGDT (média) no T1 e T2. Houve diferença no desempenho do GIN entre orelha direita e esquerda no T2. A reprodutibilidade de teste-reteste no RGDT (média) e GIN foi substancial conforme o coeficiente de correlação intraclasse. Conclusão: Há reprodutibilidade no teste RGDT quando comparada a média das frequências e no teste GIN bilateralmente.

The behavioral test is frequent clinical practice in audiology due to its contribution to the diagnosis and speech therapy intervention processes. Objective: To verify the reproducibility of temporal resolution assessment protocols in adults. Method: A total of 34 subjects, 22 females and 12 males, with an average age of 26.21 years (20 to 52 years old; dp = 8.92) were included, following the criteria: absence of otological and/or audiological history and school complaints; normality in the audiological pattern and in the dichotic test of digits. The Random Gap Detection and Gap in Noise tests, at 50 dB, were used. Both were applied in two moments; the second application with a one-week interval of the first. The Wilcoxon test was used to analyze the performance of the sample in the GIN test in the ear and Friedman test in order to analyze the RGDT as a function of the frequency tested in the two moments. The level of significance of 5% was adopted. The intraclass correlation coefficient was used in the analysis of the agreement between the test (T1) and retest (T2) applications by the same evaluator (reproducibility). Results: There was no difference between the frequencies tested in the RGDT (mean) in T1 and T2. There was a difference in GIN performance between the ears in T2. The test-retest reproducibility in the RGDT (mean) and GIN was substantial according to the intraclass correlation coefficient. Conclusion: There is reproducibility in the RGDT test when compared to the mean frequencies and in the GIN test bilaterally.

El uso de pruebas comportamentales es una práctica clínica frecuente debido a su contribución al diagnóstico y intervención del lenguaje y del habla. Objetivo: Verificar la reproducibilidad de los protocolos de evaluación de La resolución temporal en adultos. Método: Participaron de la amuestra 34 sujetos, 22 del sexo femenino y 12 del masculino, con un promedio de 26,21 años (20 a 52 años; dp= 8,92) siguiendo los criterios: ausencia de histórico otológico, audiológico y quejas escolares; normalidad del patrón audiológico y en la prueba dicótica de dígitos. Se utilizaron las pruebas Random Gap Detection Test y Gap in Noise, a 50dB. Ambos fueron aplicados en dos momentos, siendo la segunda aplicación con intervalo de una semana de la primera. La prueba Wilcoxon fue utilizada para analizar el rendimiento de la muestra en la prueba GIN en función de la oreja y la prueba de Friedman para el análisis del RGDT en función de la frecuencia probada en los dos momentos. Se adoptó el nivel de significancia del 5%. El coeficiente de correlación intraclase fue utilizado en el análisis de la concordancia entre las aplicaciones test(T1) y reprueba(T2) por el mismo evaluador. Resultados: No hubo diferencia entre las frecuencias probadas en el RGDT en el T1 y T2. Hubo diferencias en rendimiento del GIN entre las orejas en el T2. La reproducibilidad de prueba-reprueba en el RGDT y GIN fue sustancial conforme el coeficiente de correlación intraclase. Conclusión: Hay reproducibilidad en la prueba RGDT cuando se compara el promedio de las frecuencias y la prueba GIN bilateralmente.

Influence of the socieconomic level on the temporal resolution hearing skills in adults / Influência do nível socioeconômico na habilidade auditiva de resolução temporal em adultos

ABSTRACT Objective: to investigate the influence of the socioeconomic level on the temporal resolution auditory ability of adults. Methods: the sample consisted of 48 subjects aged 18 to 50 years, divided into three groups: G1, 11 subjects at level A; G2, 19 in B1 and B2 and G3, 18 in C1, C2, D and E. All subjects presented responses in 20 dB HL in frequencies of 500 to 4000 Hz, during audiometric screening, type A tympanometry, presence of contralateral and ipsilateral acoustic reflexes, no neurological, psychiatric and / or psychological changes diagnosed; without audiological and / or otological complaints and with performance above 95% in the dichotic digit test. The Random Gap Detection and Gap-in-noise tests were performed. The Shapiro-Wilk test for normality analysis and the Kruskall Wallis test for socioeconomic stratum analysis, were used, both with 5% significance. Results: there were statistically significant differences between the groups in the Random Gap Detection in the frequency of 500 Hz and in the average of the frequencies, as a function of the socioeconomic level, the same not being observed in the Gap-in-noise. Conclusions: it is suggested that the socioeconomic level be taken into account in the analysis of the Random Gap Detection test.

RESUMO Objetivo: investigar a influência do nível socioeconômico na habilidade auditiva de resolução temporal de adultos. Métodos: a amostra foi composta por 48 sujeitos na faixa-etária de 18 a 50 anos, (X = 25,40 ± 7,36) divididos por estratos socioeconômicos em três grupos: G1: 11 sujeitos no nível A; G2: 19 no B1 e B2 e G3: 18 no C1, C2, D e E. Todos os sujeitos apresentaram respostas em 20 dB NA nas frequências de 500 a 4000Hz durante a triagem audiométrica, timpanometria tipo A, presença de reflexos acústicos contra e ipsilaterais, não possuíam alterações neurológicas, psiquiátricas e/ou psicológicas diagnosticadas; sem queixas audiológicas e/ou otológicas e com desempenho superior a 95% no teste dicótico de dígitos. Foram realizados os testes Random Gap Detection e o Gap-in-noise. Utilizou-se o teste Shapiro-Wilk para análise de normalidade e o teste de Kruskall Wallis para análise do estrato socioeconômico, ambos com 5% de significância. Resultados: houve diferença estatisticamente significante entre os grupos no Random Gap Detection na frequência de 500 Hz e na média das frequências em função do nível socioeconômico, não sendo observado o mesmo no Gap-in-noise. Conclusões: sugere-se que o nível socioeconômico seja levado em consideração na análise do teste Random Gap Detection.